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cellamp trademark direct rna prep kit  (TaKaRa)


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    Structured Review

    TaKaRa cellamp trademark direct rna prep kit
    Cellamp Trademark Direct Rna Prep Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellamp+direct+rna+prep+kit/CellAmp+Direct+RNA+Prep+Kit+for+RT-PCR/us12584132-150-5-13
    Average 95 stars, based on 172 article reviews
    cellamp trademark direct rna prep kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Downregulation of HepaRG Cell CYP Genes by Hypoxia Inducible Factor Prolyl Hydroxylase (HIF-PH) Inhibitor.
    Article Snippet: .. The medium, with or without test compounds, was replaced every 24 h. Cell Lysate for RT-PCR HepaRG cell lysates were prepared using a CellAmp Direct RNA Prep Kit for RT-PCR (TaKaRa Bio, Shiga, Japan). ..

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Article Title: Overexpression of the BRC repeat 8 of BRCA2 hyperstabilizes RAD51 and alters DNA repair dynamics
    Article Snippet: Proteins were detected using a C-DiGit Blot Scanner (Li-COR, Lincoln, Nebraska, USA), and band intensities were quanti ed using Image Studio 6.0 software (Li-COR). .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) RNA was extracted using the CellAmp Direct RNA Prep Kit (Takara Bio Inc., Shiga, Japan) following the manufacturer's instructions. qRT-PCR was performed to measure RAD51 mRNA levels using the following primers targeting RAD51: CTGATGAGTTTGGTGTAGCAG (forward) and GGAAGACAGGGAGAGTCGTAGA (reverse). ..

    Gene Expression:

    Article Title: Comparative virulence analysis of seven diverse strains of Orientia tsutsugamushi reveals a multifaceted and complex interplay of virulence factors responsible for disease.
    Article Snippet: .. Host gene expression was determined by extracting total RNA from BMDM and human primary macrophages using the CellAmp Direct RNA Prep Kit (Takara, cat# 3732, Japan), following the manufacturer’s instructions. .. RNA was transcribed and amplified using the One Step TB Green PrimeScript RT-PCR Kit (Takara, cat# RR066A, Japan).

    Article Title: Comparative virulence analysis of seven diverse strains of Orientia tsutsugamushi reveals a multifaceted and complex interplay of virulence factors responsible for disease
    Article Snippet: .. Host gene expression was determined by extracting total RNA from BMDM and human primary macrophages using the CellAmp Direct RNA Prep Kit (Takara, cat# 3732, Japan), following the manufacturer’s instructions. .. RNA was transcribed and amplified using the One Step TB Green PrimeScript RT-PCR Kit (Takara, cat# RR066A, Japan).

    Lysis:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Synthesized:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube Differentiation
    Article Snippet: .. Cell lysis was performed, and cDNA was synthesized at 37 °C for 30 min using a Thermal Cycler Dice Touch (Takara Bio, Shiga, Japan) and CellAmp Direct RNA Prep Kit for RT-PCR (Takara Bio, Japan) following the manufacturer’s protocol. qPCR from cDNA, TB Green Fast qPCR Mix (Takara Bio, Japan), and thermal cycling programs in the user manual were followed. .. The primer sequences used for qPCR are summarized in Table S2 ., Forty cycles were applied for qPCR using an Applied Biosystems 7500 RT System or Step-One (Applied Biosystems, USA).

    Quantitative RT-PCR:

    Article Title: Overexpression of the BRC repeat 8 of BRCA2 hyperstabilizes RAD51 and alters DNA repair dynamics
    Article Snippet: Proteins were detected using a C-DiGit Blot Scanner (Li-COR, Lincoln, Nebraska, USA), and band intensities were quanti ed using Image Studio 6.0 software (Li-COR). .. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) RNA was extracted using the CellAmp Direct RNA Prep Kit (Takara Bio Inc., Shiga, Japan) following the manufacturer's instructions. qRT-PCR was performed to measure RAD51 mRNA levels using the following primers targeting RAD51: CTGATGAGTTTGGTGTAGCAG (forward) and GGAAGACAGGGAGAGTCGTAGA (reverse). ..

    Article Title: Ferroptotic Neutrophils Induce Immunosuppression and Chemoresistance in Breast Cancer
    Article Snippet: .. With the use of the CellAmp Direct RNA Prep Kit for qRT-PCR KIT (TaKaRa), total RNA was extracted from isolated T cells or neutrophils. .. Next, using the SYBR Premix Ex Taq kit (TaKaRa) in accordance with the manufacturer’s instructions, qRT-PCR was performed.

    other:

    Article Title: Schisandrin B exerts anti-colorectal cancer effect through CXCL2/ERK/DUSP11 signaling pathway
    Article Snippet: The CellAmpTM Direct RNA Prep Kit, PrimeScript RT Master Mix, and SYBR ® Premix Ex TaqTM were obtained from Takara (Beijing, China).

    Isolation:

    Article Title: Ferroptotic Neutrophils Induce Immunosuppression and Chemoresistance in Breast Cancer
    Article Snippet: .. With the use of the CellAmp Direct RNA Prep Kit for qRT-PCR KIT (TaKaRa), total RNA was extracted from isolated T cells or neutrophils. .. Next, using the SYBR Premix Ex Taq kit (TaKaRa) in accordance with the manufacturer’s instructions, qRT-PCR was performed.



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